Publication: An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine
Issued Date
2002-06-19
Resource Type
ISSN
0001706X
Other identifier(s)
2-s2.0-0036272534
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Tropica. Vol.83, No.1 (2002), 49-61
Suggested Citation
Oumaporn Tasanor, Harald Noedl, Kesara Na-Bangchang, Kanungnit Congpuong, Jeeraphat Sirichaisinthop, Walther H. Wernsdorfer An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine. Acta Tropica. Vol.83, No.1 (2002), 49-61. doi:10.1016/S0001-706X(02)00056-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20206
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Title
An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine
Abstract
Following earlier observations on short-term culture of Plasmodium vivax, an in vitro test system has been developed for assessing the parasite's sensitivity to chloroquine. Fresh isolates with predominantly young trophozoites are diluted 1:19 with a (v/v=1/1) mixture of RPMI 1640 and Waymouth medium. The blood-medium mixture (BMM) is inoculated into the predosed microtitre plates before incubation in a candle jar. Incubation for 30 or 42 h yielded the best results. Incubation for 18 or 24 h was generally insufficient for an adequate development of the parasites. The reading of the test is based on stage-specific differential counts in the Giemsa-stained pre-incubation and post-incubation thick films, the evaluation on log-probit analysis of drug-related inhibition of parasite development. The test system has been evaluated on 200 fresh P. vivax isolates in an area with satisfactory clinical-parasitological response to chloroquine. At 30 or 42 h incubation 121 isolates (61.5%) showed adequate control growth and yielded valid sensitivity tests. Complete inhibition of parasite development occurred within the concentration range of 40-1280 nM. The mean EC50for 30 h of incubation was 50.3 nM, as compared to 49.7 nM with 42 h of incubation. The geometric mean cut-off concentration of parasite development was 488 nM with 30 h of incubation as against 470 nM with 42 h of incubation. © 2002 Elsevier Science Ireland Ltd. All rights reserved.