Publication: Photorhabdus luminescens subsp. Namnaonensis subsp. nov., isolated from Heterorhabditis baujardi nematodes
Issued Date
2017-04-01
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14665026
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2-s2.0-85019049436
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Mahidol University
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SCOPUS
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International Journal of Systematic and Evolutionary Microbiology. Vol.67, No.4 (2017), 1046-1051
Suggested Citation
Stefanie P. Glaeser, Nicholas J. Tobias, Aunchalee Thanwisai, Narisara Chantratita, Helge B. Bode, Peter Kämpfer Photorhabdus luminescens subsp. Namnaonensis subsp. nov., isolated from Heterorhabditis baujardi nematodes. International Journal of Systematic and Evolutionary Microbiology. Vol.67, No.4 (2017), 1046-1051. doi:10.1099/ijsem.0.001761 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41584
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Photorhabdus luminescens subsp. Namnaonensis subsp. nov., isolated from Heterorhabditis baujardi nematodes
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Abstract
© 2017 IUMS. A lightly yellowish-pigmented, oxidase-negative bacterial strain (PB45.5T) isolated from the Nam Nao district of Phetchabun in central Thailand was investigated to determine its taxonomic position. Cells of the isolate showed a rod shaped appearance. The strain stained Gram-negative. Strain PB45.5Tshared highest 16S rRNA gene sequence similarity with the type strains of Photorhabdus luminescens subsp. akhurstii (99.2 %) and Photorhabdus luminescens subsp. hainanensis (99.1 %) and lower similarities to all other Photorhabdus luminescens subspecies (<98.0 %). Multilocus sequence analysis (MLSA) based on concatenated partial recA, dnaN, gltX, gyrB and infB gene sequences confirmed the affiliation obtained by 16S rRNA gene sequence analysis but showed a clear distinction of PB45.5Tfrom the closest related type strains. Strain PB45.5Tshared only 96.9% sequence similarity (concatenated nucleotide sequences) with P. luminescens subsp. akhurstii FRG04Tand 96.8% with P. luminescens subsp. hainanensis C8404T. The fatty acid profile of the strain consisted of the major fatty acids C14: 0, C16: 0, C17: 0cyclo, C16: 1ω7c and/or iso-C15: 02-OH, and C18: 1ω7c. The MLSA results and the differential biochemical and chemotaxonomic properties showed that strain PB45.5Trepresents a novel P. luminescens subspecies, for which the name Photorhabdus luminescens subsp. namnaonensis subsp. nov. (type strain PB45.5T=LMG 29915T=CCM 8729T) is proposed.