Publication:
Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance

dc.contributor.authorYusuke Ohnoen_US
dc.contributor.authorJarunya Narangajavanaen_US
dc.contributor.authorAkiko Yamamotoen_US
dc.contributor.authorTsukaho Hattorien_US
dc.contributor.authorYasuaki Kagayaen_US
dc.contributor.authorJerzy Paszkowskien_US
dc.contributor.authorWilhelm Gruissemen_US
dc.contributor.authorLars Hennigen_US
dc.contributor.authorShin Takedaen_US
dc.contributor.otherNagoya Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherMie Universityen_US
dc.contributor.otherUniversite de Geneveen_US
dc.contributor.otherETH Zurichen_US
dc.contributor.otherSveriges lantbruksuniversiteten_US
dc.date.accessioned2018-05-03T08:00:42Z
dc.date.available2018-05-03T08:00:42Z
dc.date.issued2011-09-01en_US
dc.description.abstractChromatin reconstitution after DNA replication and repair is essential for the inheritance of epigenetic information, but mechanisms underlying such a process are still poorly understood. Previously, we proposed that Arabidopsis BRU1 functions to ensure the chromatin reconstitution. Loss-of-function mutants of BRU1 are hypersensitive to genotoxic stresses and cause release of transcriptional gene silencing of heterochromatic genes. In this study, we show that BRU1 also plays roles in gene regulation in euchromatic regions. bru1 mutations caused sporadic ectopic expression of genes, including those that encode master regulators of developmental programs such as stem cell maintenance and embryogenesis. bru1 mutants exhibited adventitious organogenesis, probably due to the misexpression of such developmental regulators. The key regulatory genes misregulated in bru1 alleles were often targets of PcG SET-domain proteins, although the overlap between the bru1-misregulated and PcG SET-domain-regulated genes was limited at a genome-wide level. Surprisingly, a considerable fraction of the genes activated in bru1 were located in several subchromosomal regions ranging from 174 to 944 kb in size. Our results suggest that BRU1 has a function related to the stability of subchromosomal gene regulation in the euchromatic regions, in addition to the maintenance of chromatin states coupled with heritable epigenetic marks. © 2011 by the Genetics Society of America.en_US
dc.identifier.citationGenetics. Vol.189, No.1 (2011), 83-95en_US
dc.identifier.doi10.1534/genetics.111.130062en_US
dc.identifier.issn19432631en_US
dc.identifier.issn00166731en_US
dc.identifier.other2-s2.0-80052671543en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/11480
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052671543&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleEctopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenanceen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052671543&origin=inwarden_US

Files

Collections