Publication: In vitro study of parasite elimination and endothelial protection by curcumin: Adjunctive therapy for cerebral malaria
Issued Date
2014-03-20
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ISSN
16112156
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2-s2.0-84897054383
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Mahidol University
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SCOPUS
Bibliographic Citation
EXCLI Journal. Vol.13, (2014), 287-299
Suggested Citation
Sarun Kunwittaya, Lertyot Treeratanapiboon, Apapan Srisarin, Chartchalerm Isarankura-Na-Ayudhya, Virapong Prachayasittikul In vitro study of parasite elimination and endothelial protection by curcumin: Adjunctive therapy for cerebral malaria. EXCLI Journal. Vol.13, (2014), 287-299. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33033
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Title
In vitro study of parasite elimination and endothelial protection by curcumin: Adjunctive therapy for cerebral malaria
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Abstract
Plasmodium falciparum infection can abruptly progress to severe malaria and cerebral malaria. Despite the current efficiency of antimalarial drugs in killing parasites, no specific effective treatment has been found for cerebral malaria. Thus, a new strategy targeting both parasite elimination and endothelial cell protection is urgently needed in this field. In this study, we determined whether curcumin, which has blood-brain permeability, antioxidative activity and/or immunomodulation property, provided a potential effect on both parasite elimination and endothelial protection. Murine brain microvascular endothelial cells (bEnd.3; ATCC) were cocultured with Plasmodium falciparum-infected red blood cells (Pf-IRBC), peripheral blood mononuclear cell (PBMC) and platelets. Apoptosis of endothelial cells was demonstrated by annexin V staining. Interestingly, curcumin exhibited high efficiency of antimalarial activity (IC50~10 μM) and decreased bEnd.3 apoptosis down to 60.0 % and 79.6 % upon pretreatment and co-treatment, respectively, with Pf-IRBC, platelets and PBMC. Our findings open up a high feasibility of applying curcumin as a potential adjunctive compound for cerebral malaria treatment in the future.