Publication: Evaluation of the diagnostic accuracy of an affordable rapid diagnostic test for African Swine Fever antigen detection in Lao People's Democratic Republic
Issued Date
2020-12-01
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ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-85091377972
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.286, (2020)
Suggested Citation
Nina Matsumoto, Jarunee Siengsanan-Lamont, Laurence J. Gleeson, Bounlom Douangngeun, Watthana Theppangna, Syseng Khounsy, Phouvong Phommachanh, Tariq Halasa, Russell D. Bush, Stuart D. Blacksell Evaluation of the diagnostic accuracy of an affordable rapid diagnostic test for African Swine Fever antigen detection in Lao People's Democratic Republic. Journal of Virological Methods. Vol.286, (2020). doi:10.1016/j.jviromet.2020.113975 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/59108
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Title
Evaluation of the diagnostic accuracy of an affordable rapid diagnostic test for African Swine Fever antigen detection in Lao People's Democratic Republic
Abstract
© 2020 The Authors African Swine Fever (ASF) is a transboundary animal disease of pigs and wild suids that appeared in Lao People's Democratic Republic (Lao PDR) in mid-2019, having spread across China and Vietnam in the months prior. Despite the scale of the Asian ASF pandemic and the availability of pen-side rapid diagnostic tests (RDT) on the market, few locally produced and easily available ASF RDTs have been evaluated for diagnostic accuracy. In this study, an ASF antigen detection RDT from Shenzhen Lvshiyuan Biotechnology Co. Ltd was evaluated using clinical field samples submitted to the National Animal Health Laboratory (NAHL) from ASF suspect cases between June and December 2019 in Lao PDR. Positive (n = 57) and negative (n = 50) samples of whole blood, serum and haemolysed serum were assessed by RDT and PCR, with the latter used as the gold standard reference comparator. Overall the RDT had a diagnostic sensitivity (DSe) of 65 %, 95 % CI [51–77] and diagnostic specificity (DSp) of 76 %, 95 % CI [62–87]. The RDT demonstrated improved performance on samples with lower PCR cycle threshold (ct) values with each additional cycle reducing the odds of the RDT returning a positive by 17 % relative to the previous cycle, 95 % CI [8 %–28 %] (P < 0.01). While this test shows promise for field application, complete validation of diagnostic accuracy requires a larger sample size.