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Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

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dc.contributor.authorSomsin Petyimen_US
dc.contributor.authorChanon Neungtonen_US
dc.contributor.authorIsarin Thanaboonyawaten_US
dc.contributor.authorPitak Laokirkkiaten_US
dc.contributor.authorRoungsin Choavaratanaen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-11-09T01:55:49Z
dc.date.available2018-11-09T01:55:49Z
dc.date.issued2014-01-01en_US
dc.description.abstract© 2014, Springer Science+Business Media New York. Purpose: To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation.Methods: Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group.Result (s): The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P < 0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P < 0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P < 0.001).Conclusion (s): Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.en_US
dc.identifier.citationJournal of Assisted Reproduction and Genetics. Vol.31, No.12 (2014), 1673-1680en_US
dc.identifier.doi10.1007/s10815-014-0332-yen_US
dc.identifier.issn15737330en_US
dc.identifier.issn10580468en_US
dc.identifier.other2-s2.0-84914159575en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14594/33346
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84914159575&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectMedicineen_US
dc.titleSperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparationen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84914159575&origin=inwarden_US
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