Publication: Transcriptional regulation of iNOS and COX-2 by a novel compound from Curcuma comosa in lipopolysaccharide-induced microglial activation
Issued Date
2009-09-22
Resource Type
ISSN
03043940
Other identifier(s)
2-s2.0-67651114149
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Neuroscience Letters. Vol.462, No.2 (2009), 171-175
Suggested Citation
Anusorn Thampithak, Yamaratee Jaisin, Benjawan Meesarapee, Sukumal Chongthammakun, Pawinee Piyachaturawat, Piyarat Govitrapong, Porntip Supavilai, Yupin Sanvarinda Transcriptional regulation of iNOS and COX-2 by a novel compound from Curcuma comosa in lipopolysaccharide-induced microglial activation. Neuroscience Letters. Vol.462, No.2 (2009), 171-175. doi:10.1016/j.neulet.2009.06.094 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28301
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Transcriptional regulation of iNOS and COX-2 by a novel compound from Curcuma comosa in lipopolysaccharide-induced microglial activation
Abstract
Overproduction of pro-inflammatory mediators resulting from chronic activation of microglia has been implicated in many neurodegenerative disorders, such as Parkinson's disease and Alzheimer's disease. In this study, we investigated the effects of (3R) 1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol, or compound 049 on the production of pro-inflammatory mediators in lipopolysaccharide (LPS)-treated microglia. Compound 049 is a pure compound fractionated from the hexane extract of Curcuma comosa, an indigenous plant of Thailand traditionally used as an anti-inflammatory agent for the treatment of uterine inflammation. It was found that pretreatment of the highly aggressively proliferating immortalized (HAPI), rat microglial cell line, with compound 049, at the concentrations of 0.1, 0.5 and 1 μM significantly decreased LPS-induced NO and PGE2production in a concentration-dependent manner. Parallel to the decreases in NO and PGE2production was a reduction in the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2) as measured by mRNA and protein levels. These results indicate that compound 049 possesses an anti-inflammatory activity and may have a therapeutic potential for the treatment of neurodegenerative diseases related to microglial activation. © 2009 Elsevier Ireland Ltd. All rights reserved.