Publication: Inhibition of p38MAPK and CD137 signaling reduce dengue virus-induced TNF-α secretion and apoptosis
Issued Date
2013
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Language
eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Virology Journal. Vol. 10, (2013), 105
Suggested Citation
Amar Nagila, Janjuree Netsawang, Aroonroong Suttitheptumrong, Atthapan Morchang, Sasiprapa Khunchai, Chatchawan Srisawat, Chunya Puttikhunt, Sansanee Noisakran, Pa-thai Yenchitsomanus, Thawornchai Limjindaporn Inhibition of p38MAPK and CD137 signaling reduce dengue virus-induced TNF-α secretion and apoptosis. Virology Journal. Vol. 10, (2013), 105. doi:10.1186/1743-422X-10-105 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2649
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Title
Inhibition of p38MAPK and CD137 signaling reduce dengue virus-induced TNF-α secretion and apoptosis
Abstract
Background: Hepatic injury in dengue virus (DENV) infection is authenticated by hepatomegaly and an upsurge in
transaminase levels. DENV replicates in hepatocytes and causes hepatocyte apoptosis both in vitro and in vivo.
Understanding the molecular mechanisms of DENV-induced hepatic injury could facilitate the development of
alternate chemotherapeutic agents and improved therapies.
Findings: The p38 mitogen-activated protein kinase (MAPK) participates in both apoptosis-related signaling and
pro- inflammatory cytokine production. The role of p38 MAPK in DENV-infected HepG2 cells was examined using
RNA interference. The results showed that DENV infection activated p38 MAPK and induced apoptosis. The p38
MAPK activation and TNF-α production were controlled by p38 MAPK and CD137 signaling in DENV-infected
HepG2 cells as activated p38 MAPK, TNF-α and apoptosis were significantly decreased in p38 MAPK and CD137
depleted DENV-infected HepG2 cells. Addition of exogenous TNF-α to p38 MAPK depleted DENV-infected HepG2
cells restored DENV-induced apoptosis in HepG2 cells.
Conclusion: DENV induces CD137 signaling to enhance apoptosis by increasing TNF-α production via activation of
p38 MAPK.