Publication:
Fasciola gigantica, F. Hepatica and Fasciola intermediate forms: Geometric morphometrics and an artificial neural network to help morphological identification

dc.contributor.authorSuchada Sumruaypholen_US
dc.contributor.authorPraphaiphat Siribaten_US
dc.contributor.authorJean Pierre Dujardinen_US
dc.contributor.authorSébastien Dujardinen_US
dc.contributor.authorChalit Komalamisraen_US
dc.contributor.authorUrusa Thaenkhamen_US
dc.contributor.otherUniversité de Montpellieren_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2020-05-05T04:59:33Z
dc.date.available2020-05-05T04:59:33Z
dc.date.issued2020-01-01en_US
dc.description.abstractCopyright 2020 Sumruayphol et al. Background. Fasciola hepatica and F. gigantica cause fascioliasis in both humans and livestock. Some adult specimens of Fasciola sp. referred to as ‘‘intermediate forms’’ based on their genetic traits, are also frequently reported. Simple morphological criteria are unreliable for their specific identification. In previous studies, promising phenotypic identification scores were obtained using morphometrics based on linear measurements (distances, angles, curves) between anatomical features. Such an approach is commonly termed ‘‘traditional’’ morphometrics, as opposed to ‘‘modern’’ morphometrics, which is based on the coordinates of anatomical points. Methods. Here, we explored the possible improvements that modern methods of morphometrics, including landmark-based and outline-based approaches, could bring to solving the problem of the non-molecular identification of these parasites. F. gigantica and Fasciola intermediate forms suitable for morphometric characterization were selected from Thai strains following their molecular identification. Specimens of F. hepatica were obtained from the Liverpool School of Tropical Medicine (UK). Using these three taxa, we tested the taxonomic signal embedded in traditional linear measurements versus the coordinates of anatomical points (landmark- and outline-based approaches). Various statistical techniques of validated reclassification were used, based on either the shortest Mahalanobis distance, the maximum likelihood, or the artificial neural network method. Results. Our results revealed that both traditional and modern morphometric approaches can help in the morphological identification of Fasciola sp. We showed that the accuracy of the traditional approach could be improved by selecting a subset of characters among the most contributive ones. The influence of size on discrimination by shape was much more important in traditional than in modern analyses. In our study, the modern approach provided different results according to the type of data: satisfactory when using pseudolandmarks (outlines), less satisfactory when using landmarks. The different reclassification methods provided approximately similar scores, with a special mention to the neural network, which allowed improvements in accuracy by combining data from both morphometric approaches. Conclusion. We conclude that morphometrics, whether traditional or modern, represent a valuable tool to assist in Fasciola species recognition. The general level of accuracy is comparable among the various methods, but their demands on skills and time differ. Based on the outline method, our study could provide the first description of the shape differences between species, highlighting the more globular contours of the intermediate forms.en_US
dc.identifier.citationPeerJ. Vol.2020, No.2 (2020)en_US
dc.identifier.doi10.7717/peerj.8597en_US
dc.identifier.issn21678359en_US
dc.identifier.other2-s2.0-85083567940en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/54451
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083567940&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectNeuroscienceen_US
dc.titleFasciola gigantica, F. Hepatica and Fasciola intermediate forms: Geometric morphometrics and an artificial neural network to help morphological identificationen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083567940&origin=inwarden_US

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