Publication: Pseuderanthemum palatiferum (Nees) radlk extract induces apoptosis via reactive oxygen species-mediated mitochondria-dependent pathway in A549 human lung cancer cells
9
Issued Date
2019-02-01
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ISSN
15969827
15965996
15965996
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2-s2.0-85063650160
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Mahidol University
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SCOPUS
Bibliographic Citation
Tropical Journal of Pharmaceutical Research. Vol.18, No.2 (2019), 287-294
Suggested Citation
Udomlak Kongprasom, Wanida Sukketsiri, Pennapa Chonpathompikunlert, Morakot Sroyraya, Somporn Sretrirutchai, Supita Tanasawet Pseuderanthemum palatiferum (Nees) radlk extract induces apoptosis via reactive oxygen species-mediated mitochondria-dependent pathway in A549 human lung cancer cells. Tropical Journal of Pharmaceutical Research. Vol.18, No.2 (2019), 287-294. doi:10.4314/tjpr.v18i2.10 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/51911
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Title
Pseuderanthemum palatiferum (Nees) radlk extract induces apoptosis via reactive oxygen species-mediated mitochondria-dependent pathway in A549 human lung cancer cells
Abstract
© 2019 The authors. Purpose: To investigate the capacity of aqueous Pseuderanthemum paltiferum leaf extracts (PPA) to induce apoptosis in A549 human lung cancer cells and the possible mechanisms of action. Methods: Human lung cancer A549 cells were cultured in the presence of PPA (0-1000 µg/mL). Cell viability was assessed by MTT assay while morphological alterations in the cells were observed by Hoechst 33342/PI double staining. Intracellular reactive oxygen species (ROS) levels and subsequent changes of mitochondrial membrane potential were also investigated. Involvement of caspase-3 activation in the apoptotic pathway was determined. Results: PPA inhibited the growth of A549 cells in a concentration-and time-dependent manner. Major phenotypic apoptotic cell death was evidenced in microscopic images. Furthermore, treatment of A549 cells with PPA resulted in a significant increase in the production of ROS accompanied by attenuation of mitochondrial membrane potential, thus inducing the activation of caspase-3 activity (p < 0.05). Conclusion: PPA exerts anti-cancer activity by suppression of cell viability and induction of ROS-mediated mitochondrial dependent apoptosis in A549 cells, and may be a potential candidate for the development of a therapeutic agent for lung cancer.