Publication: In vitro analysis of the anticancer activity of Lysinibacillus sphaericus binary toxin in human cancer cell lines
Issued Date
2020-08-01
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ISSN
21905738
2190572X
2190572X
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2-s2.0-85088782591
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Mahidol University
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SCOPUS
Bibliographic Citation
3 Biotech. Vol.10, No.8 (2020)
Suggested Citation
Wasutorn Chankamngoen, Tavan Janvilisri, Boonhiang Promdonkoy, Panadda Boonserm In vitro analysis of the anticancer activity of Lysinibacillus sphaericus binary toxin in human cancer cell lines. 3 Biotech. Vol.10, No.8 (2020). doi:10.1007/s13205-020-02361-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57590
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Title
In vitro analysis of the anticancer activity of Lysinibacillus sphaericus binary toxin in human cancer cell lines
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Abstract
© 2020, King Abdulaziz City for Science and Technology. Binary or Bin toxin produced by Lysinibacillus sphaericus is composed of BinA (42 kDa) and BinB (51 kDa) subunits. These work together to exert maximal toxicity against mosquito larvae via pore formation and induction of apoptosis. The C-terminal domains in both subunits are homologous to those of aerolysin-type β pore-forming toxins, including parasporin-2 (PS2). The latter is one of the Bacillus thuringiensis toxins that exhibits specific cytotoxicity against human cancer cells. The present study investigates the possible anticancer activity of Bin toxin using PS2 as a control. We demonstrate that treatment with a high concentration of trypsin-activated Bin inhibits cell proliferation in human cancer cells A549, Caco-2, HepG2, HK-1 and KKU-M055. In the most susceptible cells, HK-1, Bin toxin exposure led to morphological alterations, decreased migration, decreased adhesion activity and apoptosis induction. Although these effects necessitated high concentrations, they suggest that Bin toxin may be optimized as a novel potential cancer-therapeutic agent.