Publication: Rapid and sensitive detection of Laem-Singh virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick
Issued Date
2011-10-01
Resource Type
ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-84860413913
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.177, No.1 (2011), 71-74
Suggested Citation
Narong Arunrut, Yortyot Seetang-Nun, Jurairat Phromjai, Wattana Panphut, Wansika Kiatpathomchai Rapid and sensitive detection of Laem-Singh virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. Journal of Virological Methods. Vol.177, No.1 (2011), 71-74. doi:10.1016/j.jviromet.2011.06.020 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11982
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Title
Rapid and sensitive detection of Laem-Singh virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick
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Abstract
Laem-Singh virus (LSNV) was discovered recently in Thailand in farmed Giant Tiger shrimp (Penaeus monodon) displaying signs of slow growth syndrome. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here a reverse transcription (RT)-LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect LSNV RNA rapidly and specifically. The reaction was optimized at 65 °C for 30. min and amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5. min was detected at LFD test line 5. min after application. Including 10. min for rapid RNA extraction, test results could be generated within 1. h and did not require electrophoresis. Compared to an existing RT-PCR method, the RT-LAMP-LFD was also ∼1000-fold more sensitive in detecting LSNV RNA. © 2011 Elsevier B.V.