Publication: The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles
Issued Date
2013-09-15
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ISSN
14779129
09501991
09501991
Other identifier(s)
2-s2.0-84883233566
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Mahidol University
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SCOPUS
Bibliographic Citation
Development (Cambridge). Vol.140, No.18 (2013), 3819-3825
Suggested Citation
Ai Khim Lim, Chanchao Lorthongpanich, Ting Gang Chew, Chin Wee Godwin Tan, Yan Ting Shue, Sathish Balu, Natalia Gounko, Satomi Kuramochi-Miyagawa, Martin M. Matzuk, Shinichiro Chuma, Daniel M. Messerschmidt, Davor Solter, Barbara B. Knowles The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles. Development (Cambridge). Vol.140, No.18 (2013), 3819-3825. doi:10.1242/dev.099184 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31215
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Title
The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles
Abstract
Mobilization of endogenous retrotransposons can destabilize the genome, an imminent danger during epigenetic reprogramming of cells in the germline. The P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA) pathway is known to silence retrotransposons in the mouse testes. Several piRNA pathway components localize to the unique, germline structure known as the nuage. In this study, we surveyed mouse ovaries and found, for the first time, transient appearance of nuage-like structures in oocytes of primordial follicles. Mouse vasa homolog (MVH), Piwi-like 2 (PIWIL2/MILI) and tudor domain-containing 9 (TDRD9) are present in these structures, whereas aggregates of germ cell protein with ankyrin repeats, sterile alpha motif and leucine zipper (GASZ) localize separately in the cytoplasm. Retrotransposons are silenced in primordial ovarian follicles, and de-repressed upon reduction of piRNA expression in Mvh, Mili or Gasz mutants. However, these null-mutant females, unlike their male counterparts, are fertile, uncoupling retrotransposon activation from sterility. © 2013. Published by The Company of Biologists Ltd.