Publication: Homosubtypic and heterosubtypic antibodies against highly pathogenic avian influenza H5N1 recombinant proteins in H5N1 survivors and non-H5N1 subjects
Issued Date
2014-01-01
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ISSN
10960341
00426822
00426822
Other identifier(s)
2-s2.0-84896504414
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Mahidol University
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SCOPUS
Bibliographic Citation
Virology. Vol.454-455, No.1 (2014), 254-262
Suggested Citation
Pirom Noisumdaeng, Phisanu Pooruk, Jarunee Prasertsopon, Susan Assanasen, Rungrueng Kitphati, Prasert Auewarakul, Pilaipan Puthavathana Homosubtypic and heterosubtypic antibodies against highly pathogenic avian influenza H5N1 recombinant proteins in H5N1 survivors and non-H5N1 subjects. Virology. Vol.454-455, No.1 (2014), 254-262. doi:10.1016/j.virol.2014.02.024 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34025
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Title
Homosubtypic and heterosubtypic antibodies against highly pathogenic avian influenza H5N1 recombinant proteins in H5N1 survivors and non-H5N1 subjects
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Abstract
Six recombinant vaccinia viruses containing HA, NA, NP, M or NS gene insert derived from a highly pathogenic avian influenza H5N1 virus, and the recombinant vaccinia virus harboring plasmid backbone as the virus control were constructed. The recombinant proteins were characterized for their expression and subcellular locations in TK-cells. Antibodies to the five recombinant proteins were detected in all 13 sequential serum samples collected from four H5N1 survivors during four years of follow-up; and those directed to rVac-H5 HA and rVac-NA proteins were found in higher titers than those directed to the internal proteins as revealed by indirect immunofluorescence assay. Although all 28 non-H5N1 subjects had no neutralizing antibodies against H5N1 virus, they did have cross-reactive antibodies to those five recombinant proteins. A significant increase in cross-reactive antibody titer to rVac-H5 HA and rVac-NA was found in paired blood samples from patients infected with the 2009 pandemic virus. © 2014 Elsevier Inc.