Publication: Changes in the levels, expression, and possible roles of serotonin and dopamine during embryonic development in the giant freshwater prawn, Macrobrachium rosenbergii
Issued Date
2016-01-01
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ISSN
10956840
00166480
00166480
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2-s2.0-84949530614
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Mahidol University
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SCOPUS
Bibliographic Citation
General and Comparative Endocrinology. Vol.225, (2016), 71-80
Suggested Citation
Yotsawan Tinikul, Jaruwan Poljaroen, Ruchanok Tinikul, Prasert Sobhon Changes in the levels, expression, and possible roles of serotonin and dopamine during embryonic development in the giant freshwater prawn, Macrobrachium rosenbergii. General and Comparative Endocrinology. Vol.225, (2016), 71-80. doi:10.1016/j.ygcen.2015.09.018 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43158
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Title
Changes in the levels, expression, and possible roles of serotonin and dopamine during embryonic development in the giant freshwater prawn, Macrobrachium rosenbergii
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Abstract
© 2015 Elsevier Inc. We investigated the changes in the levels of serotonin (5-HT) and dopamine (DA), and their possible roles during embryonic development of the freshwater prawn, Macrobrachium rosenbergii. The 5-HT and DA concentrations were quantified using high performance liquid chromatography with electrochemical detection (HPLC-ECD). The levels of 5-HT and DA gradually increased from early developing embryos to late developing embryos. The 5-HT concentrations gradually increased from the pale yellow egg to orange egg stages, and reaching a maximum at the black egg stage. DA concentrations were much lower in the early embryos than those of 5-HT (P<0.05), and gradually increased to reach the highest level at the black egg stage. Immunohistochemically, 5-HT was firstly detected in the early embryonic stages, whereas DA developed later than 5-HT. Functionally, 5-HT-treated female prawns at doses of 2.5×10-5, 2.5×10-6and 2.5×10-7mol/prawn, produced embryos with significantly shortened lengths of early embryonic stages, whereas DA-treated prawns at all three doses, exerted its effects by significantly lengthening the period of mid-embryonic stage onwards. These results suggest significant involvement of 5-HT and DA in embryonic developmental processes of this species.