Publication: Detection of MNS hybrid molecules in the Thai population using PCR-SSP technique
Issued Date
2007-06-01
Resource Type
ISSN
13653148
09587578
09587578
Other identifier(s)
2-s2.0-34250175845
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Transfusion Medicine. Vol.17, No.3 (2007), 169-174
Suggested Citation
P. Palacajornsuk, O. Nathalang, S. Tantimavanich, S. Bejrachandra, M. E. Reid Detection of MNS hybrid molecules in the Thai population using PCR-SSP technique. Transfusion Medicine. Vol.17, No.3 (2007), 169-174. doi:10.1111/j.1365-3148.2007.00747.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24543
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Title
Detection of MNS hybrid molecules in the Thai population using PCR-SSP technique
Abstract
We developed a polymerase chain reaction-sequence-specific primer (PCR-SSP) technique to screen for hybrid molecules in the MNS blood group in the Thai population using two sets of newly designed primers specific for four GYP(B-A-B) hybrids, GP.Mur, GP.Hop, GP.Bun and GP.HF, and two GYP(A-B-A) hybrids, GP.Vw and GP.Hut. One thousand and forty-one blood samples were tested with human anti-Mia by conventional tube technique, and 598 samples of these were tested by the PCR-SSP technique. Ninety-four samples (9.03%) were strongly positive with human antisera by conventional tube technique. For PCR-SSP test results, the GP.Hut, GP.Mur, GP.Hop, GP.Bun and GP.HF genotypes were amplified with the first set of primers, whereas GP.Vw genotype was amplified with a second set of primers. The GYP(A-B) hybrids (GP.Hil and GP.JL), GYP(A-B-A) hybrids (GP.Nob, GP.Joh and GP.Dane), GYPA, GYPB and GYPE were not amplified by either set of primers. Results of testing 94 Mi(a+) and 504 Mi(a-) by conventional tube technique and PCR-SSP were concordant. This study shows that analysis by PCR-SSP is simple and convenient; therefore, it can be used as an alternative to conventional tube technique for mass screening for MNS hybrids, especially when specific antisera are not available. © 2007 British Blood Transfusion Society.