Publication: Chiral chromatographic method to determine the enantiomers of halofantrine and its main chiral desbutyl metabolite in erythrocytes
Issued Date
1998-08-07
Resource Type
ISSN
15726495
Other identifier(s)
2-s2.0-0032493708
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Chromatography B: Biomedical Applications. Vol.712, No.1-2 (1998), 259-262
Suggested Citation
Emmanuelle Gorichon, Corinne Martin, Kesara Na Bangchang, Juntra Karbwang, Alain Thuillier, Robert Farinotti, François Gimenez Chiral chromatographic method to determine the enantiomers of halofantrine and its main chiral desbutyl metabolite in erythrocytes. Journal of Chromatography B: Biomedical Applications. Vol.712, No.1-2 (1998), 259-262. doi:10.1016/S0378-4347(98)00151-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18342
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Chiral chromatographic method to determine the enantiomers of halofantrine and its main chiral desbutyl metabolite in erythrocytes
Other Contributor(s)
Abstract
We describe a direct liquid chromatographic method with spectrofluorimetric detection to quantify the two enantiomers of halofantrine and the two enantiomers of its main chiral N-monodesbutylated metabolite in erythrocyte pellets. The method involves a Chiralpak AD column and a rapid one-step extraction procedure with acetonitrile. The method was validated for the four enantiomers within the range 0-1000 ng/ml. The absence of stereoconversion was studied in samples stored frozen for up to eight months. The optical rotation of the halofantrine and metabolite enantiomers was determined after separation on a semi-preparative Chiralcel OD column with polarimetric detection.