Publication: Detection of H5N1 avian influenza virus from mosquitoes collected in an infected poultry farm in Thailand
Issued Date
2008-02-01
Resource Type
ISSN
15303667
Other identifier(s)
2-s2.0-39449127942
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Mahidol University
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SCOPUS
Bibliographic Citation
Vector-Borne and Zoonotic Diseases. Vol.8, No.1 (2008), 105-109
Suggested Citation
Philippe Barbazan, Arunee Thitithanyanont, Dorothée Missé, Audrey Dubot, Priscille Bosc, Natsuang Luangsri, Jean Paul Gonzalez, Pattamaporn Kittayapong Detection of H5N1 avian influenza virus from mosquitoes collected in an infected poultry farm in Thailand. Vector-Borne and Zoonotic Diseases. Vol.8, No.1 (2008), 105-109. doi:10.1089/vbz.2007.0142 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19375
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Title
Detection of H5N1 avian influenza virus from mosquitoes collected in an infected poultry farm in Thailand
Abstract
Blood-engorged mosquitoes were collected at poultry farms during an outbreak of highly pathogenic avian influenza in Central Thailand during October 2005. These mosquitoes tested positive for H5N1 virus by reverse transcription-polymerase chain reaction (RT-PCR). Results were confirmed by limited sequencing of the H5 and N1 segments. Infection and replication of this virus in the C636 mosquito cell line was confirmed by quantitative real-time PCR. However, transmission by mosquitoes was not evaluated, and further research is needed. Collecting and testing mosquitoes engorged with the blood of domestic or wild animals could be a valuable tool for veterinary and public health authorities who conduct surveillance for H5N1 virus spread. © 2008 Mary Ann Liebert, Inc.