Publication:
Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae

dc.contributor.authorChontida Tangsongcharoenen_US
dc.contributor.authorNusara Chomaneeen_US
dc.contributor.authorBoonhiang Promdonkoyen_US
dc.contributor.authorPanadda Boonsermen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand National Center for Genetic Engineering and Biotechnologyen_US
dc.date.accessioned2018-11-23T09:30:42Z
dc.date.available2018-11-23T09:30:42Z
dc.date.issued2015-06-01en_US
dc.description.abstract© 2015 Elsevier Inc. During sporulation, a Gram-positive bacterium Lysinibacillus sphaericus (. Ls) produces the mosquito larvicidal binary toxin composed of 2 subunits, BinA and BinB. Full toxicity against Culex and Anopheles mosquito larvae is achieved when both subunits are administered together at equimolar amounts. Although cellular responses to Bin toxin have been reported in previous studies, it remains essential to extensively examine the cytopathic effects in vivo to define the underlying mechanism of larval death. In this study, 4th instar Culex quinquefasciatus larvae fed with different doses of Bin toxin were analyzed both for ultrastructural as well as biochemical effects. Typical morphological changes consistent with apoptosis were observed in mosquito larvae exposed to Bin toxin, including mitochondrial swelling, chromatin condensation, cytoplasmic vacuolization and apoptotic cell formation. Bin toxin also induced the activation of caspase-9 and caspase-3 in larval midgut cells. Our current observations thus suggest that Bin toxin triggers apoptosis via an intrinsic or mitochondrial pathway in vivo, possibly contributing to larval death.en_US
dc.identifier.citationJournal of Invertebrate Pathology. Vol.128, (2015), 57-63en_US
dc.identifier.doi10.1016/j.jip.2015.04.008en_US
dc.identifier.issn10960805en_US
dc.identifier.issn00222011en_US
dc.identifier.other2-s2.0-84929468594en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/35153
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929468594&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleLysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvaeen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929468594&origin=inwarden_US

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