Publication: Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification
| dc.contributor.author | Chalalai Rueanghiran | en_US |
| dc.contributor.author | Chamnarn Apiwathnasorn | en_US |
| dc.contributor.author | Pradit Sangthong | en_US |
| dc.contributor.author | Yudthana Samung | en_US |
| dc.contributor.author | Jiraporn Ruangsittichai | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Kasetsart University | en_US |
| dc.date.accessioned | 2018-05-03T08:21:23Z | |
| dc.date.available | 2018-05-03T08:21:23Z | |
| dc.date.issued | 2011-12-01 | en_US |
| dc.description.abstract | DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species. | en_US |
| dc.identifier.citation | Southeast Asian Journal of Tropical Medicine and Public Health. Vol.42, No.6 (2011), 1381-1387 | en_US |
| dc.identifier.issn | 01251562 | en_US |
| dc.identifier.other | 2-s2.0-84857668658 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/12177 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84857668658&origin=inward | en_US |
| dc.subject | Medicine | en_US |
| dc.title | Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification | en_US |
| dc.type | Review | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84857668658&origin=inward | en_US |