Publication: Biotinylated probes for epidemiological studies of drug resistance in salmonella krefeld
Issued Date
1990-04-01
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ISSN
03057453
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2-s2.0-0025324262
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Antimicrobial Chemotherapy. Vol.25, No.4 (1990), 593-603
Suggested Citation
A. Pilantanapak, A. Bhumiratana, P. Jayanetra, W. Panbangred Biotinylated probes for epidemiological studies of drug resistance in salmonella krefeld. Journal of Antimicrobial Chemotherapy. Vol.25, No.4 (1990), 593-603. doi:10.1093/jac/25.4.593 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16059
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Title
Biotinylated probes for epidemiological studies of drug resistance in salmonella krefeld
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Abstract
A gene probe for ampicillin resistance and one for sulphonamide resistance were prepared to study the origin and the relation of multiple drug resistances in Salmonella krefeld. The resistance genes were cloned into the pACYC184 vector of Escherichia coli from a common plasmid of S. krefeld that encoded for resistance to ampicillin, chloramphenicol, kanamycin, streptomycin, sulphonamide and tetracycline resistance. Restriction map analysis and deletion analysis of a recombinant plasmid (pACSS1) showed that the gene determining ampicillin resistance was located on a 1·34 and 1·12 kb PstI fragment, and that the gene for sulphonamide resistance was located on a 0·85 kb PstI fragment. These fragments were used as probes. Their specificity was tested by colony hybridization with various bacterial species, including sensitive and resistance S. krefeld isolates. Further study indicated that the ampicillin resistance gene probe reacted with the gene for TEM-1β-lactamase and that the gene probe for sulphonamide resistance reacted with the gene for type II dihydropteroate synthase. The two probes were sufficiently specific to allow study of the epidemiology of resistance in S. krefeld and other enteric bacteria. © 1990 The British Society for Antimicrobial Chemotherapy.
