Publication: Separation of diethylpyrocarbonate-modified histidine-containing peptides using strong cation exchange chromatography
Issued Date
2021-09-01
Resource Type
ISSN
01253395
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2-s2.0-85122289106
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Mahidol University
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SCOPUS
Bibliographic Citation
Songklanakarin Journal of Science and Technology. Vol.43, No.5 (2021), 1443-1448
Suggested Citation
Pongsakorn Thawonpan, Supinya Thanapongpichat, Watthanachai Jumpathong, Aung Win Tun, Churat Weeraphan, Worrawit Wanitsuwan, Hansuk Buncherd Separation of diethylpyrocarbonate-modified histidine-containing peptides using strong cation exchange chromatography. Songklanakarin Journal of Science and Technology. Vol.43, No.5 (2021), 1443-1448. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79305
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Title
Separation of diethylpyrocarbonate-modified histidine-containing peptides using strong cation exchange chromatography
Abstract
This work focuses on the separation of diethylpyrocarbonate-modified histidine-containing peptides by strong cation exchange (SCX) chromatography prior to mass spectrometric analysis. The decrease in positive charge of diethylpyrocarbonate-treated imidazole in histidine, masked by the incorporation of the carbethoxyl group, provides us with an opportunity to use SCX for separating diethylpyrocarbonate-modified histidine-containing peptides from the unmodified ones. In this report, the diethylpyrocarbonate-modified histidine-containing peptide is eluted faster than the untreated peptide. Furthermore, the carbethoxy group can be effectively removed to generate the unmodified imidazole in the histidine residue by neutral hydroxylamine.