Publication: Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models
Issued Date
2010-05-10
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ISSN
10313613
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2-s2.0-77951784898
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Mahidol University
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SCOPUS
Bibliographic Citation
Reproduction, Fertility and Development. Vol.22, No.5 (2010), 788-795
Suggested Citation
Duangjai Boonkusol, Andras Dinnyes, Tassanee Faisaikarm, Parisatcha Sangsuwan, Nathnapith Pratipnatalang, Mayurachat Sa-Ardrit, Kulnasan Saikhun, Saovaros Svasti, Jim Vadolas, Pranee Winichagoon, Suthat Fucharoen, Yindee Kitiyanant Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models. Reproduction, Fertility and Development. Vol.22, No.5 (2010), 788-795. doi:10.1071/RD09128 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28505
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Title
Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models
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Abstract
The purpose of the present study was to investigate the efficiency of embryo cryopreservation for four transgenic (TG) thalassaemic mouse strains, which is a key element of the ongoing gene banking efforts for these highvalue animals. Heterozygous TG embryos were produced by breeding four lines of TG males to wild-type (WT) females (C57BL/6J). Intact two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates of cryopreserved embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast, the paternal line had a significant effect on the development of these embryos to the blastocyst stage, which ranged from 50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from 17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced byTG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed from vitrified-warmed embryos showed expression of the human β-globin transgene. In conclusion, the present study shows a strongTG line effect on developmental competence following cryopreservation and the vitrification method was successful to bank the human β-globin TG-expressing mouse strains. © 2010 CSIRO.