In vitro cytokine changes after pediatric liver transplantation

Abstract

© 2015,Allergy and Immunology Society of Thailand. All rights reserved. Background: Patients with chronic liver disease have been shown to have impaired immune statuses. Liver transplantation (LT) is the standard treatment for end-stage liver disease patients and immunosuppressive drugs are commonly used to prevent graft rejection. There is an increasing evidence of de novo food allergies post LT. Objective: To investigate the cytokine response of peripheral blood mononuclear cells (PBMCs) of pediatric LT recipients before and six months after transplantation. Method: PBMCs collected before and six months after LT were stimulated with phytohemagglutinin (PHA), beta-lactoglobulin (BLG), tacrolimus (Tac), dexamethasone (Dex), and a combination of BLG and Dex (B+D), BLG and Tac (B+T), BLG and Tac plus Dex (B+T+D). Culture supernatants were measured for IL-5, IFN-γ and IL-10. Blood for liver function tests, complete blood counts, total IgE and specific IgE (sIgE) to cow’s milk were recorded. Results: A total of five pediatric LT recipients were enrolled in the study. There were no food allergy cases. Total IgE and sIgE to cow’s milk decreased significantly after LT. After transplantation, there was a significant increase in IL-5, IFN- γ and IL-10 in culture supernatants of PHA-stimulated PBMCs. Among different stimulations in post transplantation’s PBMCs, the level of IL-5 significantly increased in B+D and B+T stimulated cells. However, this effect was suppressed with the combination of B+T+D. The level of IL-10 significantly increased in all conditions containing BLG both before and after transplantation. Conclusion: There was an improvement of the in vitro- cytokine responses after liver transplantations. Immunosuppressive drugs used in post transplantation had an effect on the cytokine responses.