Publication: Identification of Blastocystis subtype 1 variants in the home for girls, Bangkok, Thailand
Issued Date
2013-02-01
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ISSN
00029637
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2-s2.0-84874046452
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.88, No.2 (2013), 352-358
Suggested Citation
Umaporn Thathaisong, Suradej Siripattanapipong, Mathirut Mungthin, Duangnate Pipatsatitpong, Peerapan Tan-Ariya, Tawee Naaglor, Saovanee Leelayoova Identification of Blastocystis subtype 1 variants in the home for girls, Bangkok, Thailand. American Journal of Tropical Medicine and Hygiene. Vol.88, No.2 (2013), 352-358. doi:10.4269/ajtmh.2012.12-0237 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31967
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Title
Identification of Blastocystis subtype 1 variants in the home for girls, Bangkok, Thailand
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Abstract
A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.