Publication:
Blood transcriptomics to characterize key biological pathways and identify biomarkers for predicting mortality in melioidosis

Issued Date

2021-01-01

Resource Type

Article

ISSN

22221751

DOI

10.1080/22221751.2020.1858176

Other identifier(s)

2-s2.0-85099684166

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Emerging Microbes and Infections. Vol.10, No.1 (2021), 8-18

Suggested Citation

Thatcha Yimthin, Jacqueline Margaret Cliff, Rungnapa Phunpang, Peeraya Ekchariyawat, Taniya Kaewarpai, Ji Sook Lee, Clare Eckold, Megan Andrada, Ekkachai Thiansukhon, Kittisak Tanwisaid, Somchai Chuananont, Chumpol Morakot, Narongchai Sangsa, Wirayut Silakun, Sunee Chayangsu, Noppol Buasi, Nicholas Day, Ganjana Lertmemongkolchai, Wasun Chantratita, T. Eoin West, Narisara Chantratita Blood transcriptomics to characterize key biological pathways and identify biomarkers for predicting mortality in melioidosis. Emerging Microbes and Infections. Vol.10, No.1 (2021), 8-18. doi:10.1080/22221751.2020.1858176 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77358

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Blood transcriptomics to characterize key biological pathways and identify biomarkers for predicting mortality in melioidosis

Author(s)

Thatcha Yimthin
 Jacqueline Margaret Cliff
 Rungnapa Phunpang
 Peeraya Ekchariyawat
 Taniya Kaewarpai
 Ji Sook Lee
 Clare Eckold
 Megan Andrada
 Ekkachai Thiansukhon
 Kittisak Tanwisaid
 Somchai Chuananont
 Chumpol Morakot
 Narongchai Sangsa
 Wirayut Silakun
 Sunee Chayangsu
 Noppol Buasi
 Nicholas Day
 Ganjana Lertmemongkolchai
 Wasun Chantratita
 T. Eoin West
 Narisara Chantratita

Other Contributor(s)

Udon Thani Center Hospital
 London School of Hygiene & Tropical Medicine
 Surin Hospital
 Khon Kaen University
 Faculty of Medicine Ramathibodi Hospital, Mahidol University
 Imperial College London
 Mahidol University
 Nuffield Department of Medicine
 John A. Burns School of Medicine
 Harborview Medical Center
 Chiang Mai University
 Sisaket Hospital
 Roi Et Hospital
 Nakhon Phanom Hospital
 Mukdahan Hospital
 Buriram Hospital

Abstract

Melioidosis is an often lethal tropical disease caused by the Gram-negative bacillus, Burkholderia pseudomallei. The study objective was to characterize transcriptomes in melioidosis patients and identify genes associated with outcome. Whole blood RNA-seq was performed in a discovery set of 29 melioidosis patients and 3 healthy controls. Transcriptomic profiles of patients who did not survive to 28 days were compared with patients who survived and healthy controls, showing 65 genes were significantly up-regulated and 218 were down-regulated in non-survivors compared to survivors. Up-regulated genes were involved in myeloid leukocyte activation, Toll-like receptor cascades and reactive oxygen species metabolic processes. Down-regulated genes were hematopoietic cell lineage, adaptive immune system and lymphocyte activation pathways. RT-qPCR was performed for 28 genes in a validation set of 60 melioidosis patients and 20 healthy controls, confirming differential expression. IL1R2, GAS7, S100A9, IRAK3, and NFKBIA were significantly higher in non-survivors compared with survivors (P < 0.005) and healthy controls (P < 0.0001). The AUROCC of these genes for mortality discrimination ranged from 0.80-0.88. In survivors, expression of IL1R2, S100A9 and IRAK3 genes decreased significantly over 28 days (P < 0.05). These findings augment our understanding of this severe infection, showing expression levels of specific genes are potential biomarkers to predict melioidosis outcomes.

Keyword(s)

Immunology and Microbiology
 Medicine
 Pharmacology, Toxicology and Pharmaceutics

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/77358

Collections

Scopus 2021