Publication:
Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections

Issued Date

2021-12-01

Resource Type

Article

ISSN

20452322

DOI

10.1038/s41598-021-01052-5

Other identifier(s)

2-s2.0-85118684781

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Scientific Reports. Vol.11, No.1 (2021)

Suggested Citation

Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Paul G. Higgins, Cameron Hurst, Shuichi Abe, Parichart Hongsing, Naphat Chantaravisoot, Thammakorn Saethang, Sirirat Luk-in, Tingting Liao, Sumanee Nilgate, Ubolrat Rirerm, Naris Kueakulpattana, Matchima Laowansiri, Sukrit Srisakul, Netchanok Muhummudaree, Teerasit Techawiwattanaboon, Lin Gan, Chenchen Xu, Rosalyn Kupwiwat, Phatthranit Phattharapornjaroen, Rojrit Rojanathanes, Asada Leelahavanichkul, Tanittha Chatsuwan Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-01052-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79200

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Title

Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections

Author(s)

Aye Mya Sithu Shein
 Dhammika Leshan Wannigama
 Paul G. Higgins
 Cameron Hurst
 Shuichi Abe
 Parichart Hongsing
 Naphat Chantaravisoot
 Thammakorn Saethang
 Sirirat Luk-in
 Tingting Liao
 Sumanee Nilgate
 Ubolrat Rirerm
 Naris Kueakulpattana
 Matchima Laowansiri
 Sukrit Srisakul
 Netchanok Muhummudaree
 Teerasit Techawiwattanaboon
 Lin Gan
 Chenchen Xu
 Rosalyn Kupwiwat
 Phatthranit Phattharapornjaroen
 Rojrit Rojanathanes
 Asada Leelahavanichkul
 Tanittha Chatsuwan

Other Contributor(s)

Yamagata Prefectural Central Hospital
 Medizinische Fakultät
 Mae Fah Luang University Hospital
 UWA Medical School
 Jiangsu Province Hospital
 Thammasat University Hospital
 Chulalongkorn University
 Sahlgrenska Akademin
 QIMR Berghofer Medical Research Institute
 Kasetsart University
 Mae Fah Luang University
 Faculty of Medicine Ramathibodi Hospital, Mahidol University
 Mahidol University
 Partner Site Bonn-Cologne
 Fuling Center Hospital of Chongqing City

Abstract

Development of an effective therapy to overcome colistin resistance in Klebsiella pneumoniae, a common pathogen causing catheter-related biofilm infections in vascular catheters, has become a serious therapeutic challenge that must be addressed urgently. Although colistin and EDTA have successful roles for eradicating biofilms, no in vitro and in vivo studies have investigated their efficacy in catheter-related biofilm infections of colistin-resistant K. pneumoniae. In this study, colistin resistance was significantly reversed in both planktonic and mature biofilms of colistin-resistant K. pneumoniae by a combination of colistin (0.25–1 µg/ml) with EDTA (12 mg/ml). This novel colistin-EDTA combination was also demonstrated to have potent efficacy in eradicating colistin-resistant K. pneumoniae catheter-related biofilm infections, and eliminating the risk of recurrence in vivo. Furthermore, this study revealed significant therapeutic efficacy of colistin-EDTA combination in reducing bacterial load in internal organs, lowering serum creatinine, and protecting treated mice from mortality. Altered in vivo expression of different virulence genes indicate bacterial adaptive responses to survive in hostile environments under different treatments. According to these data discovered in this study, a novel colistin-EDTA combination provides favorable efficacy and safety for successful eradication of colistin-resistant K. pneumonia catheter-related biofilm infections.

Keyword(s)

Multidisciplinary

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/79200

Collections

Scopus 2021