Publication: Detection of human immunoglobulin G by transmission surface plasmon resonance using the in situ gold nanoparticle growth method
2
Issued Date
2019-02-01
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ISSN
17451353
09168524
09168524
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2-s2.0-85061122639
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Mahidol University
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SCOPUS
Bibliographic Citation
IEICE Transactions on Electronics. Vol.E102C, No.2 (2019), 125-131
Suggested Citation
Theerasak Juagwon, Chutiparn Lertvachirapaiboon, Kazunari Shinbo, Keizo Kato, Toemsak Srikhirin, Tanakorn Osotchan, Akira Baba Detection of human immunoglobulin G by transmission surface plasmon resonance using the in situ gold nanoparticle growth method. IEICE Transactions on Electronics. Vol.E102C, No.2 (2019), 125-131. doi:10.1587/transele.2018OMP0004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50862
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Title
Detection of human immunoglobulin G by transmission surface plasmon resonance using the in situ gold nanoparticle growth method
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Abstract
Copyright © 2019 The Institute of Electronics, Information and Communication Engineers. In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.