Publication: Deferiprone increases endothelial nitric oxide synthase phosphorylation and nitric oxide production
Issued Date
2018-01-01
Resource Type
ISSN
12057541
00084212
00084212
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2-s2.0-85052672314
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Mahidol University
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SCOPUS
Bibliographic Citation
Canadian Journal of Physiology and Pharmacology. Vol.96, No.9 (2018), 879-885
Suggested Citation
Thanaporn Sriwantana, Pornpun Vivithanaporn, Kittiphong Paiboonsukwong, Krit Rattanawonsakul, Sirada Srihirun, Nathawut Sibmooh Deferiprone increases endothelial nitric oxide synthase phosphorylation and nitric oxide production. Canadian Journal of Physiology and Pharmacology. Vol.96, No.9 (2018), 879-885. doi:10.1139/cjpp-2018-0012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45276
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Title
Deferiprone increases endothelial nitric oxide synthase phosphorylation and nitric oxide production
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Abstract
© 2018, Canadian Science Publishing. All rights reserved. Iron chelation can improve endothelial function. However, effect on endothelial function of deferiprone has not been reported. We hypothesized deferiprone could promote nitric oxide (NO) production in endothelial cells. We studied effects of deferiprone on blood nitrite and blood pressure after single oral dose (25 mg/kg) in healthy subjects and hemoglobin E/β-thalassemia patients. Further, effects of deferiprone on NO production and endothelial NO synthase (eNOS) phosphorylation in primary human pulmonary artery endothelial cells (HPAEC) were investigated in vitro. Blood nitrite levels were higher in patients with deferiprone therapy than those without deferiprone (P = 0.023, n = 16 each). Deferiprone increased nitrite in plasma and whole blood of healthy subjects (P = 0.002 and 0.044) and thalassemia patients (P = 0.003 and 0.046) at time 180 min (n = 20 each). Asymptomatic reduction in diastolic blood pressure (P = 0.005) and increase in heart rate (P = 0.009) were observed in healthy subjects, but not in thalassemia patients. In HPAEC, deferiprone increased cellular nitrite and phospho-eNOS (Ser1177) (P = 0.012 and 0.035, n = 6) without alteration in total eNOS protein and mRNA. We conclude that deferiprone can induce NO production by enhancing eNOS phosphorylation in endothelial cells.