Publication:
The effect of temperature and UV light on infectivity of avian influenza virus (H<inf>5</inf>N<inf>1</inf>, Thai field strain) in chicken fecal manure

1

Issued Date

2006-01-01

Resource Type

Article

ISSN

01251562

Other identifier(s)

2-s2.0-33744468508

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.1 (2006), 102-105

Suggested Citation

Kamlang Chumpolbanchorn, Namphung Suemanotham, Nakrop Siripara, Bopit Puyati, Kridsada Chaichoune The effect of temperature and UV light on infectivity of avian influenza virus (H<inf>5</inf>N<inf>1</inf>, Thai field strain) in chicken fecal manure. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.1 (2006), 102-105. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/23839

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Title

The effect of temperature and UV light on infectivity of avian influenza virus (H<inf>5</inf>N<inf>1</inf>, Thai field strain) in chicken fecal manure

Author(s)

Kamlang Chumpolbanchorn
 Namphung Suemanotham
 Nakrop Siripara
 Bopit Puyati
 Kridsada Chaichoune

Other Contributor(s)

Mahidol University

Abstract

Normal chicken fecal manure (pH 8.23 and 13.7% moisture) was investigated for infectivity of the avian influenza virus (AIV; H5N1). The manure was divided into three groups; each group was inoculated with AIV at 2.38 × 105.25ELD50. After viral inoculation, the first group was incubated at 25°C. The second group was kept at 40°C, and the last group was exposed to ultraviolet light at 4-5 μw/cm2at room temperature. After incubation, a 20% suspension of manure was filtered and the filtrates were inoculated into 9-11 day-old embryonated chicken eggs per WHO protocol (2002). The results showed that at 25°C the virus lost its infectivity within 24 hours, and at 40°C within 15 minutes. UV light, however, could not destroy the infectivity of the virus even after exposure for 4 hours.

Keyword(s)

Medicine

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URI

https://repository.li.mahidol.ac.th/handle/123456789/23839

Collections

Scopus 2006-2010