Publication: Immobilized lipase from potential lipolytic microbes for catalyzing biodiesel production using palm oil as feedstock
Issued Date
2011-02-01
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ISSN
16845315
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2-s2.0-79952300921
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Mahidol University
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SCOPUS
Bibliographic Citation
African Journal of Biotechnology. Vol.10, No.9 (2011), 1666-1673
Suggested Citation
Pakorn Winayanuwattikun, Chutima Kaewpiboon, Kingkaew Piriyakananon, Warawut Chulalaksananukul, Tikamporn Yongvanich, Jisnuson Svasti Immobilized lipase from potential lipolytic microbes for catalyzing biodiesel production using palm oil as feedstock. African Journal of Biotechnology. Vol.10, No.9 (2011), 1666-1673. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11359
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Title
Immobilized lipase from potential lipolytic microbes for catalyzing biodiesel production using palm oil as feedstock
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Abstract
Biodiesel has been regarded as a biodegradable and non-polluting fuel. Enzymatic transesterification reaction for manufacturing biodiesel from vegetable oils with alcohol is an attractive approach. However, the cost of enzyme remains a barrier for its industrial implementation. The aim of this research was the screening of lipase-producing microorganisms and the studies of potential lipasemediated biodiesel production using palm oil as substrate. A total of 360 strains of bacteria, yeasts and fungi were isolated and screened from the samples of oil-contaminated soil and waste water. Among all the screened microbes, the potential lipolytic bacterium, Staphylococcus warneri, unicellular yeast, Candida rugosa and filamentous fungus, Fusarium solani were selected because of their high specific activities. The lipase-producing conditions were subsequently optimized by using palm oil as an inducer and lipase activities were compared for both hydrolytic and synthetic catalysis. C. rugosa lipase, which exhibited the highest potential for catalyzing the biodiesel production, was further purified and immobilized on various hydrophobic supports. The catalysis of transesterification between methanol and palm oil by the C. rugosa immobilized lipases revealed that immobilized lipase from C. rugosa on Sepabeads EC-OD was the most promising for further development as a biocatalyst for the application of enzyme-catalyzed biodiesel synthesis. © 2011 Academic Journals.