Publication: Metabolic acidosis lowers circulating adiponectin through inhibition of adiponectin gene transcription
Issued Date
2011-02-01
Resource Type
ISSN
14602385
09310509
09310509
Other identifier(s)
2-s2.0-79551491539
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nephrology Dialysis Transplantation. Vol.26, No.2 (2011), 592-598
Suggested Citation
Sinee Disthabanchong, Kannika Niticharoenpong, Piyanuch Radinahamed, Wasana Stitchantrakul, Boonsong Ongphiphadhanakul, Suradej Hongeng Metabolic acidosis lowers circulating adiponectin through inhibition of adiponectin gene transcription. Nephrology Dialysis Transplantation. Vol.26, No.2 (2011), 592-598. doi:10.1093/ndt/gfq410 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12689
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Metabolic acidosis lowers circulating adiponectin through inhibition of adiponectin gene transcription
Other Contributor(s)
Abstract
Background. Metabolic acidosis (MA) adversely affects protein and lipid metabolism as well as endocrine function. Adipose tissue communicates with the rest of the body through synthesis and release adipokines, such as leptin, adiponectin and TNF-alpha. Adiponectin enhances insulin sensitivity and possesses anti-atherogenic and anti-inflammatory properties. Circulating adiponectin correlates inversely with cardiovascular events. It is possible that MA negatively regulates adiponectin contributing to poor patient outcome. The present study investigates the effect of MA on adiponectin in vivo and in vitro.Methods. Twenty healthy female volunteers underwent a 7-day course of oral ammonium chloride (NH4Cl)-induced acidosis. Serum adiponectin was determined before and after NH4Cl ingestion. Adipocytes were differentiated from their precursors, human mesenchymal stem cells (hMSCs), in culture. Concentrated HCl was added to the media to lower pH. Adiponectin mRNA and protein were determined at 48 and 96 h by real-time RTPCR and ELISA, respectively.Results. After a 7-day course of NH4Cl, serum bicarbonate decreased significantly associated with the increase in urine ammonium and titratable acid. Adiponectin decreased significantly from 10 623 to 9723 pg/mL (P < 0.005). MA suppressed adiponectin mRNA in hMSC-derived adipocytes at 48 and 96 h (P < 0.01). The amount of adiponectin released into the culture media declined corresponding to the mRNA levels (P < 0.001). MA did not affect adipocyte triglyceride or protein content.Conclusions. MA lowered circulating adiponectin through inhibition of adiponectin gene transcription in adipocytes. © The Author 2010. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.