Publication: Blood and liver biopsy for the non-destructive screening of tilapia lake virus
Issued Date
2019-11-01
Resource Type
ISSN
13652761
01407775
01407775
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2-s2.0-85073666548
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Fish Diseases. Vol.42, No.11 (2019), 1629-1636
Suggested Citation
Chutipong Chiamkunakorn, Vimbai Irene Machimbirike, Saengchan Senapin, Pongsak Khunrae, Ha Thanh Dong, Triwit Rattanarojpong Blood and liver biopsy for the non-destructive screening of tilapia lake virus. Journal of Fish Diseases. Vol.42, No.11 (2019), 1629-1636. doi:10.1111/jfd.13076 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/49714
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Title
Blood and liver biopsy for the non-destructive screening of tilapia lake virus
Abstract
© 2019 John Wiley & Sons Ltd Detection of tilapia lake virus (TiLV) in tilapines is mainly from visceral organs of killed fish. However, lethal sampling might not be viable to broodstock and economically important ornamental cichlids. To contribute towards screening of the virus in asymptomatic infected fish, a subclinically infected population of Nile tilapia adults obtained from a local farm was preliminarily tested to compare different non-lethal sampling methods, for example liver biopsy, gill biopsy, fin clip, mucus, faeces and blood for detection of TiLV. Only liver and blood samples gave positive results by PCR. Since blood sampling is relatively simpler, it was further used for five naturally co-cultured juvenile fish species from above-mentioned farm including 40 red tilapia broodstock and 20 Nile tilapia adults from two other different farms. The results showed that from the tested fish, 4 of 5 Nile tilapia, 2 of 5 hybrid red tilapia and 3 of 5 giant gourami blood samples tested positive, while 38 of 40 blood samples of red tilapia tested positive for TiLV in second-step PCR. Sequencing representative PCR amplicons of positive samples confirmed sequence identity to TiLV. In conclusion, both blood and liver biopsy are practical non-destructive sampling platforms for TiLV screening in cichlids with blood being more convenient, especially for tilapia broodstock.