Publication: The Effect of adding L-Cysteine in Modified Kenny's extender on the quality of chilled stallion semen at 5oC
Issued Date
2013
Resource Type
Language
eng
ISSN
1906-2257
Rights
Mahidol University
Rights Holder(s)
Faculty of Veterinary Science Mahidol University
Bibliographic Citation
Journal of Applied Animal Science. Vol.6, No.1 (2013), 43-52
Suggested Citation
Panithi Sukho, Hiran Juwarahawong, Slitwan Sangiam, Kampon Kaeoket The Effect of adding L-Cysteine in Modified Kenny's extender on the quality of chilled stallion semen at 5oC. Journal of Applied Animal Science. Vol.6, No.1 (2013), 43-52. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13328
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Title
The Effect of adding L-Cysteine in Modified Kenny's extender on the quality of chilled stallion semen at 5oC
Alternative Title(s)
ผลของการใส่สารแอลซีสเทอีน (L-Cysteine) ในสารละลายเจือจางน้ำเชื้อ Modified Kenney's ในการเก็บรักษาน้ำเชื้อพ่อม้าด้วยการแช่เย็นที่อุณหภูมิ 5 องศาเซลเซียส
Abstract
The aim of this study was to investigate the effect of supplementation of L-Cysteine in Modified
Kenny's extender on chilled stallion semen quality at 5oC. Semen samples were collected from 3 stallions by
artificial vagina and centrifugation to remove seminal plasma and diluted with Modified Kenny's extender to
50 x 106 sperm/ml. Semen samples (n=10 ejaculates) were divided into 3 portions, the first portion as the control
(KE), the second portion was added with L-Cysteine 200 μmol/l (KEC200) and added L-Cysteine 400 μmol/l in
the last portion (KEC400). In all samples, the percentage of progressive motility, viability and acrosome
integrity were evaluated at 0, 24, 48 and 72 h after storage at 5oC. There were a significant (P < 0.05) lower
percentage of dead spermatozoa in group KE200 than other groups at 24 h. There was a significantly lower
percentage of progressive motility in KEC 400 compared toother groups at 48 h (P < 0.05). In addition, there
was a tendency (P = 0.1) of lower percentage of dead spermatozoa in group KE200 than other groups at 48 h.
At 72 h, comparing all groups, a higher percentage of viability in groups KEC200 and KEC400 was found,
but not statistically significant. In conclusion, Addition of L-cysteine at a concentration of 200 μmol/l to
the Modified Kenney's extender tend to improve the stallion chilled semen progressive motility, dead/alive,
viability and acrosome integrity during chilled storage at 5oC for 48 h.