Cryopreservation technique of chilli seeds by vitrification

Abstract

© 2019 International Society for Horticultural Science. All Rights Reserved. The Plant Genetic Conservation Project under the Royal Initiation of Her Royal Highness Princess Maha Chakri Sirindhorn (RSPG) has collaborated with the DOA Genebank (Genebank Research and Development Group) Biotechnology Research and Development Office, Department of Agriculture to study cryopreservation techniques of chilli seeds (Huareo no.13) by vitrification. Cryoprotectant immersion durations were tested in order to study the most suitable time for this method. Chilli seeds were immersed in loading solution containing 2 M glycerol and 0.4 M sucrose for 0, 10 and 20 min. They were then soaked in PVS2 solution containing 30% glycerol, 15% ethylene glycol 15% DMSO for 0, 30 and 60 min. After that, chilli seeds were plunged into liquid nitrogen (LN). After thawing and reculturing on MS medium + 0.5 M sucrose for 1 d, chilli seeds were taken out from the medium and transferred onto MS medium. They were then incubated for 14 d. It was found that the survival rate of chilli seeds before and after LN storage was 100%. These in vitro seedlings were successfully transplanted to field conditions.