Publication: Structure and function of HIV-1 CRF01-AE envelope proteins from blood and genital fluid isolates
Issued Date
2009-05-01
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ISSN
01251562
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2-s2.0-67650179154
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.3 (2009), 480-493
Suggested Citation
Navin Horthongkham, Niracha Athipanyasilp, Sontana Siritantikorn, Wannee Kantakamalakul, Surangrat Srisurapanon, Ruengpung Sutthent Structure and function of HIV-1 CRF01-AE envelope proteins from blood and genital fluid isolates. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.3 (2009), 480-493. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28097
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Title
Structure and function of HIV-1 CRF01-AE envelope proteins from blood and genital fluid isolates
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Abstract
The recombinant envelope protein (gpl20) of the human immunodeficiency virus type 1 (HIV-1) CRF01-AE env gene isolated from the corresponding blood (rgpl20- F36PC) and genital fluid (rgpl20-F36VC) specimens obtained from HIV infected individuals was successfully produced in both prokaryote and eukaryote cells. The yields of HIV-1 recombinant envelope proteins rgpl20-F36PC and rgpl20-F36VC produced in E. coli and in mammalian cells were 1.0 and 1.2, and 0.3 and 0.5 mg/ml, respectively. Antibody responses in mice immunized with rgpl20-F36VC protein were not significantly higher than those with rgpl20-F36PC protein. The level of antibody response in mice immunized with V3 deleted recombinant gpl20 proteins from rgpl20-F36VC and rgpl20-F36PC was not significantly different from wild type rgpl20 proteins. β- strands at the tip of the V3 loop of the HIV-1 envelope protein were predicted for the wild type genital fluid isolate but not for the wild type blood isolate. The replication capacity of both F36PC and F36VC was quite efficient. The infectivity assay of the epithelial cell line for pNL4-3/gpl20F36VC was better than for pNL4-3/gpl20F36PC. The extra β-strands in the V3 loop may be involved in cell tropism.