Publication: Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
Issued Date
2012-02-15
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ISSN
18764347
18716784
18716784
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2-s2.0-84856402141
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Mahidol University
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SCOPUS
Bibliographic Citation
New Biotechnology. Vol.29, No.3 (2012), 379-386
Suggested Citation
Suthee Benjaphokee, Daisuke Hasegawa, Daiki Yokota, Thipa Asvarak, Choowong Auesukaree, Minetaka Sugiyama, Yoshinobu Kaneko, Chuenchit Boonchird, Satoshi Harashima Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol. New Biotechnology. Vol.29, No.3 (2012), 379-386. doi:10.1016/j.nbt.2011.07.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13800
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Title
Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
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Abstract
Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridization technique without recombinant DNA technology. A heterothallic strain showing a high-temperature (41°C) tolerant (Htg + ) phenotype, a derivative from a strain isolated from nature, was crossed with a homothallic strain displaying high-ethanol productivity (Hep + ), a stock culture at the Thailand Institute of Scientific and Technological Research. The resu ltant hybrid TJ14 displayed ability to rapidly utilize glucose, and produced ethanol (46.6g/l) from 10% glucose fermentation medium at high temperature (41°C). Not only ethanol productivity at 41°C but also acid tolerance (Acd + ) was improved in TJ14 as compared with its parental strains, enabling TJ14 to grow in liquid medium even at pH 3. TJ14 maintained high ethanol productivity (46.0g/l) from 10% glucose when fermentation was done under multiple-stress conditions (41°C and pH 3.5). Furthermore, when TJ14 was subjected to a repeated-batch fermentation scheme, the growth and ethanol production of TJ14 were maintained at excellent levels over ten cycles of fermentation. Thus, the multiple-stress (Htg + Hep + Acd + ) resistant strain TJ14 should be useful for cost-effective bioethanol production under high-temperature and acidic conditions. © 2011 Elsevier B.V.