Publication: New chemiluminescence assay for linamarin
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Issued Date
1999-11-17
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ISSN
15216543
Other identifier(s)
2-s2.0-0032749988
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Mahidol University
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SCOPUS
Bibliographic Citation
IUBMB Life. Vol.48, No.2 (1999), 219-223
Suggested Citation
Sutarnthip Ruengprapavut, Montri Chulavatnatol New chemiluminescence assay for linamarin. IUBMB Life. Vol.48, No.2 (1999), 219-223. doi:10.1080/152165499307260 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25325
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Title
New chemiluminescence assay for linamarin
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Abstract
A new chemiluminescence assay was developed for the quantitative determination of linamarin, a cyanogenic glucoside present in cassava. The assay involved hydrolysis of linamarin by a specific enzyme, linamarase, to release glucose, which was then quantitated by a chemiluminescence system consisting of glucose oxidase-peroxidase-luminol. The new assay was more sensitive than the conventional spectrophotometric method for quantitating linamarin in cassava extracts. However, the following agents were found to interfere with the new assay: Vanadate, Mg2+, and Cu2+, were inhibitory to the luminescence of the H2O2-peroxidase-luminol system used in the coupling reaction, whereas EDTA and EGTA activated the system. In addition, Hg2+, which inhibits glucose oxidase, and Tris ion, which inhibits linamarase, both interfered with the new assay.