Publication: Standard Conditions of Virus Isolation Reveal Biological Variability of HIV Type 1 in Different Regions of the World
Issued Date
1994-01-01
Resource Type
ISSN
19318405
08892229
08892229
Other identifier(s)
2-s2.0-0027992804
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
AIDS Research and Human Retroviruses. Vol.10, No.11 (1994), 1401-1408
Suggested Citation
H. Rübsamen-Waigmann, H. Von Briesen, H. Holmes, Björndal, B. Korber, R. Esser, S. Ranjbar, P. Tomlinson, B. Galvao-Castro, E. Karita, S. Sempala, C. Wasi, S. Osmanov, E. M. Fenyö Standard Conditions of Virus Isolation Reveal Biological Variability of HIV Type 1 in Different Regions of the World. AIDS Research and Human Retroviruses. Vol.10, No.11 (1994), 1401-1408. doi:10.1089/aid.1994.10.1401 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9609
Research Projects
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Title
Standard Conditions of Virus Isolation Reveal Biological Variability of HIV Type 1 in Different Regions of the World
Other Contributor(s)
Georg-Speyer-Haus
National Institute for Biological Standards and Control
Institutionen For Mikrobiologi, Tumor-Och Cellbiologi
Los Alamos National Laboratory
Fundacao Oswaldo Cruz
Prins Leopold Instituut voor Tropische Geneeskunde
Uganda Virus Research Institute
Mahidol University
Organisation Mondiale de la Sante
National Institute for Biological Standards and Control
Institutionen For Mikrobiologi, Tumor-Och Cellbiologi
Los Alamos National Laboratory
Fundacao Oswaldo Cruz
Prins Leopold Instituut voor Tropische Geneeskunde
Uganda Virus Research Institute
Mahidol University
Organisation Mondiale de la Sante
Abstract
HIV-1 isolates were obtained from four countries within the framework of the WHO Network for HIV Isolation and Characterization. The use of standard HIV isolation procedures allowed us to compare the biological properties of 126 HIV-1 isolates spanning five genetic subtypes. In primary isolation cultures, viruses from Uganda and Brazil appeared early and replicated without delay, whereas the replication of Thai viruses was delayed by several weeks. Regardless of genetic subtype or country of origin, blood samples collected more than 2 years after seroconversion yielded virus that replicated efficiently in the primary isolation cultures. None of the isolates obtained from Thailand or Rwanda replicated in cell lines, whereas 5 of the 13 Brazilian isolates and 7 of the 11 Ugandan isolates replicated and induced syncytia in MT-2 cells. As expected for virus isolates obtained early in HIV-1 infection (within 2 years of seroconversion), all viruses from Brazil, Rwanda, and Thailand showed a slow/low replicative pattern. For the Ugandan samples, the time from seroconversion was known precisely for a few of the samples and only in one case was less than 2 years. This may explain why the five viruses that were able to replicate in all cell lines, and thus classified as rapid/high, were of Ugandan origin. Viruses able to induce syncytia in MT-2 cells, also induced syncytia in PBMC. However, 8 slow/low viruses (out of 27) gave discordant results, inducing syncytia in PBMC but not in MT-2 cells. Furthermore, using syncytium induction as a marker, changes in virus populations during early in vitro passage in PBMC could be observed. The results indicate that biological variation is a general property of HIV-1 in different regions of the world. Moreover, the time from HIV-1 infection, rather than genetic subtype, seems to be linked to viral phenotype. © 1994, Mary Ann Liebert, Inc. All rights reserved.