Publication: Hydroxyapatite from fish scale for potential use as bone scaffold or regenerative material
Issued Date
2016-05-01
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09284931
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2-s2.0-84961310372
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Mahidol University
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SCOPUS
Bibliographic Citation
Materials Science and Engineering C. Vol.62, (2016), 183-189
Suggested Citation
Weeraphat Pon-On, Panan Suntornsaratoon, Narattaphol Charoenphandhu, Jirawan Thongbunchoo, Nateetip Krishnamra, I. Ming Tang Hydroxyapatite from fish scale for potential use as bone scaffold or regenerative material. Materials Science and Engineering C. Vol.62, (2016), 183-189. doi:10.1016/j.msec.2016.01.051 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40603
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Hydroxyapatite from fish scale for potential use as bone scaffold or regenerative material
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Abstract
© 2016 Published by Elsevier B.V. The present paper studies the physico-chemical, bioactivity and biological properties of hydroxyapatite (HA) which is derived from fish scale (FS) (FSHA) and compares them with those of synthesized HA (sHA) obtained by co-precipitation from chemical solution as a standard. The analysis shows that the FSHA is composed of flat-plate nanocrystal with a narrow width size of about 15-20 nm and having a range of 100 nm in length and that the calcium phosphate ratio (Ca/P) is 2.01 (Ca-rich CaP). Whereas, synthesized HA consists of sub-micron HA particle having a Ca/P ratio of 1.65. Bioactivity test shows that the FSHA forms more new apatite than does the sHA after being incubated in simulated body fluid (SBF) for 7 days. Moreover, the biocompatibility study shows a higher osteoblast like cell adhesion on the FSHA surface than on the sHA substrate after 3 days of culturing. Our results also show the shape of the osteoblast cells on the FSHA changes from being a rounded shape to being a flattened shape reflecting its spreading behavior on this surface. MTT assay and ALP analysis show significant increases in the proliferation and activity of osteoblasts over the FSHA scaffold after 5 days of culturing as compared to those covering the sHA substrates. These results confirm that the bio-materials derived from fish scale (FSHA) are biologically better than the chemically synthesized HA and have the potential for use as a bone scaffold or as regenerative materials.