Publication: Isolation of infectious chikungunya virus and dengue virus using anionic polymer-coated magnetic beads
Issued Date
2013-10-01
Resource Type
ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-84879170120
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.193, No.1 (2013), 55-61
Suggested Citation
Sirilaksana Patramool, Eric Bernard, Rodolphe Hamel, Luplertlop Natthanej, Nathalie Chazal, Pornapat Surasombatpattana, Peeraya Ekchariyawat, Simon Daoust, Supatra Thongrungkiat, Frédéric Thomas, Laurence Briant, Dorothée Missé Isolation of infectious chikungunya virus and dengue virus using anionic polymer-coated magnetic beads. Journal of Virological Methods. Vol.193, No.1 (2013), 55-61. doi:10.1016/j.jviromet.2013.04.016 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31861
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Title
Isolation of infectious chikungunya virus and dengue virus using anionic polymer-coated magnetic beads
Abstract
Mosquitoes-borne viruses are a major threat for human populations. Among them, chikungunya virus (CHIKV) and dengue virus (DENV) cause thousands of cases worldwide. The recent propagation of mosquito vectors competent to transmit these viruses to temperate areas increases their potential impact on susceptible human populations. The development of sensitive methods allowing the detection and isolation of infectious viruses is of crucial interest for determination of virus contamination in humans and in competent mosquito vectors. However, simple and rapid method allowing the capture of infectious CHIKV and DENV from samples with low viral titers useful for further genetic and functional characterization of circulating strains is lacking. The present study reports a fast and sensitive isolation technique based on viral particles adsorption on magnetic beads coated with anionic polymer, poly(methyl vinyl ether-maleic anhydrate) and suitable for isolation of infectious CHIKV and DENV from the four serotypes. Starting from quite reduced biological material, this method was accurate to combine with conventional detection techniques, including qRT-PCR and immunoblotting and allowed isolation of infectious particles without resorting to a step of cultivation. The use of polymer-coated magnetic beads is therefore of high interest for rapid detection and isolation of CHIKV and DENV from samples with reduced viral loads and represents an accurate approach for the surveillance of mosquito vector in area at risk for arbovirus outbreaks. © 2013 The Authors.