Publication:
Development of three-dimensional (3D) full automated paper-based elisa devices for easy production and preservation stability

Issued Date

2015-01-01

Resource Type

Conference Paper

Other identifier(s)

2-s2.0-84983354702

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (2015), 829-831

Suggested Citation

Thanyaporn Kiatkumjorn, Kingkarn Pungjunun, Amara Apilux, Phan Trong Tue, Orawon Chailapakul, Yuzuru Takamura Development of three-dimensional (3D) full automated paper-based elisa devices for easy production and preservation stability. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (2015), 829-831. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35968

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Title

Development of three-dimensional (3D) full automated paper-based elisa devices for easy production and preservation stability

Author(s)

Thanyaporn Kiatkumjorn
 Kingkarn Pungjunun
 Amara Apilux
 Phan Trong Tue
 Orawon Chailapakul
 Yuzuru Takamura

Other Contributor(s)

Japan Advanced Institute of Science and Technology
 Chulalongkorn University
 Mahidol University

Abstract

© 15CBMS-0001. A new 3D design of paper-based device on nitrocellulose (NC) membrane with the BCIP/NBT substrate kept on soluble solid for fully automatic multistep sequencing of sandwich ELISA has been developed to improve sensitivity, mass productivity and lifetime of the device. The detection limit of the 3D device with the BCIP/NBT substrate kept on soluble solid was found to be 1.01 mIU/mL which is lower than that with direct immobilization (10.7 mIU/mL). This device can be applied for a greater variety of clinical (point of care assays) or environmental analysis.

Keyword(s)

Engineering

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/35968

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