Publication: Pinostrobin attenuates colistin-induced apoptosis of human renal proximal tubular cells
Issued Date
2021-01-01
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ISSN
25868470
25868195
25868195
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2-s2.0-85119916163
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Mahidol University
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SCOPUS
Bibliographic Citation
Pharmaceutical Sciences Asia. Vol.48, No.6 (2021), 549-556
Suggested Citation
Nichakorn Worakajit, Penjai Thongnuanjan, Napason Chabang, Sirima Soodvilai, Patoomratana Tuchinda, Sunhapas Soodvilai Pinostrobin attenuates colistin-induced apoptosis of human renal proximal tubular cells. Pharmaceutical Sciences Asia. Vol.48, No.6 (2021), 549-556. doi:10.29090/psa.2021.06.21.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/78557
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Title
Pinostrobin attenuates colistin-induced apoptosis of human renal proximal tubular cells
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Abstract
Colistin is one of the last-resort antibiotics used to treat multidrug-resistant (MDR) gram-negative bacterial infection. However, this drug causes nephrotoxicity by inducing oxidative stress and mitochondrial impairment of renal proximal tubular cells. Pinostrobin, which is a major natural bioactive compound isolated from Boesenbergia rotunda, has anti-oxidative properties and preventive effects on mitochondrial damage. Therefore, this study aimed to investigate the protective effects of pinostrobin against colistin-induced toxicity in human renal proximal tubular (RPTEC/TERTl) cells. Treatment of colistin (200 µg/ml) significantly reduced cell viability and increased apoptotic cells compared with vehicle treatment. These effects were attenuated by co-treatment with pinostrobin (50–100 µM). Colistin-induced apoptosis was correlated with increased ROS and cytochrome c expression accompanied by reduction in mitochondrial membrane potential and anti-apoptotic protein (Bcl-2) expression. These effects were abolished by co-treatment with pinostrobin. Collectively, pinostrobin has protective effects against colistin-induced apoptosis of RPTEC/TERT1 cells by improving oxidative status and mitochondrial function.