Publication: A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody
Issued Date
2018-02-01
Resource Type
ISSN
1572994X
09208569
09208569
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2-s2.0-85029801587
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Mahidol University
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SCOPUS
Bibliographic Citation
Virus Genes. Vol.54, No.1 (2018), 25-32
Suggested Citation
J. Asnet Mary, Akanitt Jittmittraphap, Siriporn Chattanadee, Pornsawan Leaungwutiwong, R. Shenbagarathai A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody. Virus Genes. Vol.54, No.1 (2018), 25-32. doi:10.1007/s11262-017-1508-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45258
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Title
A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody
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Abstract
© 2017, Springer Science+Business Media, LLC. Dengue virus (DENV) is an arthropod-borne human pathogen that represents a severe public health threat in both endemic and non-endemic regions. So far, there is no licensed vaccine or specific drugs available for dengue fever. A fifteen-amino-acid-long peptide that includes the NGR motif was chemically synthesized and conjugated with keyhole limpet hemocyanin. A standard immunization protocol was followed for the production of polyclonal antibodies by immunizing rabbits against the synthetic peptide. The immune response elicited high-titer polyclonal antibodies with the reactivity of the anti-peptide antibody against both synthetic peptide and four serotypes of DENV confirmed by DOT-ELISA. Neutralizing activity of anti-peptide antibody was found to be cross-reactive and effective resulting in 60% reduction of infectivity at 1:200 dilution in all four serotypes of DENV. Our findings have the potential to further improve our understanding of virus–host interactions and provide new insights into neutralizing antibodies and could also be used as a drug target.