Publication: Isodon rugosus (Wall. ex Benth.) codd in vitro cultures: Establishment, phytochemical characterization and in vitro antioxidant and anti-aging activities
Issued Date
2019-01-02
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ISSN
14220067
16616596
16616596
Other identifier(s)
2-s2.0-85060401301
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences. Vol.20, No.2 (2019)
Suggested Citation
Bilal Haider Abbasi, Aisha Siddiquah, Duangjai Tungmunnithum, Shankhamala Bose, Muhammad Younas, Laurine Garros, Samantha Drouet, Nathalie Giglioli-Guivarc’h, Christophe Hano Isodon rugosus (Wall. ex Benth.) codd in vitro cultures: Establishment, phytochemical characterization and in vitro antioxidant and anti-aging activities. International Journal of Molecular Sciences. Vol.20, No.2 (2019). doi:10.3390/ijms20020452 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50297
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Title
Isodon rugosus (Wall. ex Benth.) codd in vitro cultures: Establishment, phytochemical characterization and in vitro antioxidant and anti-aging activities
Abstract
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Isodon rugosus (Wall. ex Benth.) Codd accumulates large amounts of phenolics and pentacyclic triterpenes. The present study deals with the in vitro callus induction from stem and leaf explants of I. rugosus under various plant growth regulators (PGRs) for the production of antioxidant and anti-ageing compounds. Among all the tested PGRs, thidiazuron (TDZ) used alone or in conjunction with α-napthalene acetic acid (NAA) induced highest callogenesis in stem-derived explants, as compared to leaf-derived explants. Stem-derived callus culture displayed maximum total phenolic content and antioxidant activity under optimum hormonal combination (3.0 mg/L TDZ + 1.0 mg/L NAA). HPLC analysis revealed the presence of plectranthoic acid (373.92 µg/g DW), oleanolic acid (287.58 µg/g DW), betulinic acid (90.51 µg/g DW), caffeic acid (91.71 µg/g DW), and rosmarinic acid (1732.61 µg/g DW). Complete antioxidant and anti-aging potential of extracts with very contrasting phytochemical profiles were investigated. Correlation analyses revealed rosmarinic acid as the main contributor for antioxidant activity and anti-aging hyaluronidase, advance glycation end-products inhibitions and SIRT1 activation, whereas, pentacyclic triterpenoids were correlated with elastase, collagenase, and tyrosinase inhibitions. Altogether, these results clearly evidenced the great valorization potential of I. rugosus calli for the production of antioxidant and anti-aging bioactive extracts for cosmetic applications.