Publication: Antibodies in serum and bile of hamsters experimentally infected with Opisthorchis viverrini
1
Issued Date
1988-01-01
Resource Type
ISSN
00207519
Other identifier(s)
2-s2.0-0023716248
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.18, No.6 (1988), 721-727
Suggested Citation
R. Chawengkirttikul, S. Sirisinha Antibodies in serum and bile of hamsters experimentally infected with Opisthorchis viverrini. International Journal for Parasitology. Vol.18, No.6 (1988), 721-727. doi:10.1016/0020-7519(88)90111-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15562
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antibodies in serum and bile of hamsters experimentally infected with Opisthorchis viverrini
Author(s)
Other Contributor(s)
Abstract
The kinetics of antibody responses in the serum and bile of hamsters experimentally infected with either 10 or 100 Opisthorchis viverrini metacercariae were investigated using enzyme-linked immunosorbent assay (ELISA) and the antibodies were characterized by radioimmunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). IgG and IgA antibodies were detected in the serum of these animals from days 15 and 30, respectively. In the group harbouring the higher degree of infection, serum antibodies increased rapidly from day 15 to day 30 and both antibody isotypes reached a plateau level on day 30. Serum antibodies in the group with the lower degree of infection increased more slowly and did not attain a peak level until a later stage of infection (i.e. between 3 and 12 months). Unlike the serum antibodies, biliary IgG and IgA antibodies in both groups of animals did not show a significant elevation until the 12th month of infection. SDS-PAGE analyses of serum antibodies showed a strong reaction with the 89-kDa component of somatic extract while biliary antibodies also reacted strongly with other components, i.e. 73 and 62 kDa. Altogether, both the ELISA and immunoprecipitation data suggest that an infection of the hamster hepatobiliary system by a parasite that does not normally invade the host tissue can stimulate both systemic and local antibody synthesis and that the latter includes both IgG and IgA antibody isotypes. © 1988.