Publication: Cladogynos orientalis Zipp. extracts inhibit cell culture-derived hepatitis C virus genotype 2a replication in Huh-7 cells through NS5B inhibition
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Issued Date
2019-08-01
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22211691
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2-s2.0-85073911549
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Tropical Biomedicine. Vol.9, No.8 (2019), 346-352
Suggested Citation
Piyanoot Thongsri, Khanit Sa-Ngiamsuntorn, Pongtip Sithisarn, Mullika Chomnawang, Krit Thirapanmethee Cladogynos orientalis Zipp. extracts inhibit cell culture-derived hepatitis C virus genotype 2a replication in Huh-7 cells through NS5B inhibition. Asian Pacific Journal of Tropical Biomedicine. Vol.9, No.8 (2019), 346-352. doi:10.4103/2221-1691.262079 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50110
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Title
Cladogynos orientalis Zipp. extracts inhibit cell culture-derived hepatitis C virus genotype 2a replication in Huh-7 cells through NS5B inhibition
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Abstract
© 2019 Asian Pacific Journal of Tropical Biomedicine Produced by Wolters Kluwer-Medknow. Objective: To evaluate the potential anti-hepatitis C virus (HCV) activities of Cladogynos orientalis Zipp. ex Span and to investigate the molecular mode of action. Methods: Ethanolic and water extracts from various parts of Cladogynos orientalis were examined for cytotoxicity by MTT assay. Sub-cytotoxic concentrations of the extracts were used for further determining anti-HCV activity using cell culture-derived HCV genotype 2a propagated in HepaRG cell line. Immunofluorescence assay was performed to observe the effect on viruses at the pre-entry step. Mode of action at the post-entry step was investigated for the viral RNA and protein expressions by real time RT-PCR and Western blotting assays, respectively. Results: Although Cladogynos orientalis water extracts exhibited lower cytotoxicity than ethanolic extracts, all ethanolic extracts from roots, stems, and leaves of Cladogynos orientalis exhibited higher anti-HCV activities than water extracts. The highest anti-HCV activity was observed in infected cells treated with the extracts 5 h after absorption. No extracts showed pre-viral entry effect. At the post-viral entry step, only leaf ethanolic extracts inhibited NS5B expression, while all extracts did not inhibit HCV NS3 expression. Conclusions: Cladogynos orientalis ethanolic extracts could be further studied and the major active compound needs to be identified as a promising source for anti-HCV agents.