Publication: Leukaemia inhibitory factor is expressed in rat gastrocnemius muscle after contusion and increases proliferation of rat L6 myoblasts via c-Myc signalling
Issued Date
2011-08-01
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ISSN
14401681
03051870
03051870
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2-s2.0-79960729474
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical and Experimental Pharmacology and Physiology. Vol.38, No.8 (2011), 501-509
Suggested Citation
Ratchakrit Srikuea, Karyn A. Esser, Chumpol Pholpramool Leukaemia inhibitory factor is expressed in rat gastrocnemius muscle after contusion and increases proliferation of rat L6 myoblasts via c-Myc signalling. Clinical and Experimental Pharmacology and Physiology. Vol.38, No.8 (2011), 501-509. doi:10.1111/j.1440-1681.2011.05537.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11500
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Leukaemia inhibitory factor is expressed in rat gastrocnemius muscle after contusion and increases proliferation of rat L6 myoblasts via c-Myc signalling
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Abstract
1. Leukaemia inhibitory factor (LIF) has been shown to have an important role during muscle regeneration. The regenerative capacity of muscles after contusion injury in LIF-knockout mice is impaired compared with that of wild-type mice. 2. To clarify whether LIF modulates muscle regeneration by regulating myogenic precursor cell activity, we studied LIF expression and myogenic precursor cell activity in gastrocnemius muscles from Wistar rats at various times after contusion injury using immunohistochemistry and the direct effect of LIF on a rat myoblast cell line (L6). 3. After contusion injury, transient upregulation of the mRNA expression of LIF, LIF receptors and signal transducer and activator of transcription (STAT) 3, downstream of LIF and involved in enhanced cell proliferation, was observed. A marked increase in LIF protein in the cytosol of damaged myofibres was strongly correlated with a significant increase in the number of myogenic precursor cells (MyoD-positive cells) by 12h after contusion. In addition, coexpression of LIF and MyoD protein in control and injured muscles after contusion injury from 3h to 7days was evident. 4. Treatment of L6 cells with LIF (1ng/mL) in serum-free medium enhanced proliferation (bromodeoxyuridine incorporation) by 24h. This was accompanied by increased expression of c-Myc protein within 12h and was abolished by short interference RNA against c-Myc mRNA. 5. Together, the results of the present study suggest that LIF acts via paracrine and autocrine actions to regulate myogenic precursor cell activity during muscle regeneration after contusion injury and that the proliferative effect of LIF on L6 cells occurs via c-Myc signalling. © 2011 The Authors. Clinical and Experimental Pharmacology and Physiology © 2011 Blackwell Publishing Asia Pty Ltd.