Publication:
Comparison of five DNA extraction methods and optimization of a B1 gene nested PCR (nPCR) for detection of Toxoplasma gondii tissue cyst in mouse brain

1

Issued Date

2005-11-01

Resource Type

Article

ISSN

01251562

Other identifier(s)

2-s2.0-33645018343

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.6 (2005), 1377-1382

Suggested Citation

Aongart Mahittikorn, Hannes Wickert, Yaowalark Sukthana Comparison of five DNA extraction methods and optimization of a B1 gene nested PCR (nPCR) for detection of Toxoplasma gondii tissue cyst in mouse brain. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.6 (2005), 1377-1382. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16743

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Title

Comparison of five DNA extraction methods and optimization of a B1 gene nested PCR (nPCR) for detection of Toxoplasma gondii tissue cyst in mouse brain

Author(s)

Aongart Mahittikorn
 Hannes Wickert
 Yaowalark Sukthana

Other Contributor(s)

Mahidol University
 Universitat Heidelberg

Abstract

Toxoplasmosis, caused by Toxoplasma gondii, is an important parasitic disease worldwide. Different techniques have been developed for T. gondii detection. At present, polymerase chain reaction (PCR) has been widely used. However, PCR for identifying T. gondii remains unsatisfactory in many laboratories because of lack of standardization and variations in efficiency. In the present study, we optimized a nested PCR protocol (n-PCR) in order to compare the amplification of T. gondii DNA, after being extracted from mouse brain by five different DNA extraction methods including phenol chloroform, QIAamp DNA minikit, Genomic DNA purification kit and Chelex with or without proteinase K. All DNA extraction methods were able to extract DNA from a single tissue cyst from mouse brain. However, among the five DNA extraction methods, the Chelex without proteinase K appeared to be the most rapid and easiest.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/16743

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Scopus 2001-2005