Publication: Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand
Issued Date
2007-10-01
Resource Type
ISSN
09320113
Other identifier(s)
2-s2.0-34548584969
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Parasitology Research. Vol.101, No.5 (2007), 1337-1344
Suggested Citation
Atiporn Saeung, Yasushi Otsuka, Visut Baimai, Pradya Somboon, Benjawan Pitasawat, Benjawan Tuetun, Anuluck Junkum, Hiroyuki Takaoka, Wej Choochote Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand. Parasitology Research. Vol.101, No.5 (2007), 1337-1344. doi:10.1007/s00436-007-0645-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24508
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand
Other Contributor(s)
Abstract
Seventeen isolines of Anopheles barbirostris derived from animal-biting female mosquitoes showed three karyotypic forms: Form A (X2, Y1) in five isolines from Phetchaburi province; Form B (X1, X3, Y2) in three and eight isolines from Chiang Mai and Ubon Ratchathani provinces, respectively; Form C (X2, Y3) in one isoline from Phetchaburi province. All 17 isolines exhibited an average branch summation of seta 2-VI pupal skins ranging from 12.1-13.0 branches, which was in the limit of A. barbirostris (6-18 branches). Of the 12 human-biting isolines from Chiang Mai province, five isolines showed Form B (X2, Y2), and seven isolines exhibited a new karyotypic form designated as Form E (X2, Y5). All of 12 isolines had an average branch summation of seta 2-VI pupal skins ranging from 22.4-24.5 branches, which was in the limit of Anopheles campestris (17-58 branches). Thus, they were tentatively designated as A. campestris-like Forms B and E. Hybridization between A. campestris-like Forms B and E showed that they were genetically compatible, yielding viable progeny for several generations suggesting conspecific relationships of these two karyotypic forms. Reproductive isolation among crosses between A. campestris-like Form B and A. barbirostris Forms A, B, and C strongly suggested the existence of these two species. In addition, the very low intraspecific variation (genetic distance <0.005) of the nucleotide sequence of ITS2 of the rDNA and COI and COII of mitochondrial DNA of the seven isolines of A. campestris-like Forms B and E supported their conspecific relationship. The large sequence divergence of ITS2 (0.203-0.268), COI (0.026-0.032), and COII (0.030-0.038) from genomic DNA of A. campestris-like Forms B and E and the A. barbirostris Forms A, B, and C clearly supported cytogenetic and morphological evidence. © 2007 Springer-Verlag.